STATISTICS

TNF-α
H9 ( The EBV-negative T-cell line)	H9-LMP1
13.14	11.65
6.42	11.14
8.90	4.49
9.20	13.46
10.26	3.56
6.71	10.70
10.62	12.53
6.52	11.45
10.53	9.63
7.63	8.36
7.38	5.91
4.43	17.33
7.85	12.87
12.89	12.32
10.57	10.14

Statistics

1.

2. Null Hypothesis: there is no difference in TNF-α secretion between the EBV-negative T-cell line (H9) and H9-LMP1.

Alternative Hypothesis: there is a difference in TNF-α secretion between the EBV-negative T-cell line (H9) and H9-LMP1.

3. The hypothesis is two tailed because it is looking for a difference between two groups and there is no particular direction to the hypothesis (i.e. less than or more than).

4.

	H9	H9-LMP1
Mean	8.87	10.369
Standard Deviation	2.476	3.603
Standard Error	0.639	0.930
Median	6.52	11.45
Confidence Level (95.0%)

5. Graph 1 visually compares the mean of TNF-α secretion between the EBV-negative T-cell line (H9) and H9-LMP1.

From looking at the graph I can conclude that the level of TNF-α secretion is higher when LMP1 expressing vector is expressed on the T-cell line H9.

6. By looking at the graph and the mean, median and standard deviation values, a dump-bell curve can not be seen to indicate the normality of the results, therefore, a further t-test must be carried out to check for the normality of the results.

7. To make the results normally distributed I have to multiply each value by by log¹⁰ then carry out a t-test.

8.